Table 1 Characteristics of 4 commercial DENV real-time RT-PCR kits
Characteristic | Geno-sen’s | Liferiver | Realstar | Simplexa |
|---|---|---|---|---|
Target region | Not specified | Not specified | Not specified | DENV-1 NS5 |
DENV-2 NS3 | ||||
DENV-3 NS5 | ||||
DENV-4 capsid | ||||
Principle | TaqMan | TaqMan | TaqMan | TaqMan |
Dye | FAM | FAM | FAM | FAM/CFR610 |
Internal control (dye) | Yes (JOE) | Yes (JOE) | Yes (JOE) | Yes (Q670) |
PCR reactions, n | 1 | 1 | 1 | 2 |
Genotyping | No | No | No | Yes |
Quantitative | Yes | Yes | No | No |
External standards | 104–108 copies/mL | 107 copies/mL | Yes (not quantified) | Yes (not quantified) |