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Table 1 Characteristics of 4 commercial DENV real-time RT-PCR kits

From: Evaluation of four commercial real-time RT-PCR kits for the detection of dengue viruses in clinical samples

Characteristic Geno-sen’s Liferiver Realstar Simplexa
Target region Not specified Not specified Not specified DENV-1 NS5
DENV-2 NS3
DENV-3 NS5
DENV-4 capsid
Principle TaqMan TaqMan TaqMan TaqMan
Dye FAM FAM FAM FAM/CFR610
Internal control (dye) Yes (JOE) Yes (JOE) Yes (JOE) Yes (Q670)
PCR reactions, n 1 1 1 2
Genotyping No No No Yes
Quantitative Yes Yes No No
External standards 104–108 copies/mL 107 copies/mL Yes (not quantified) Yes (not quantified)