Table 3 Influenza virus tetramer staining
Animal ID/SwIV strain | Tetramer SwIV peptide | Peptide substituted from immunization strain | Frequency of tetramer (APC + BV421+) cells |
|---|---|---|---|
(Tetramer + cells subtracted negative control) | |||
ASYGAGAGY | Negative control | 0.80 (0.00) | |
1/ | CTELKLSDY | No | 1.70 (0.90) |
1 | GTEKLTITY | No | 1.90 (1.10) |
SSSFSFGGF | No | 1.70 (0.90) | |
YVFVGTSRY | No | 1.60 (0.80) | |
ASYGAGAGY | Negative control | 0.60 (0.00) | |
2/ | CTELKLSDY | No | 1.70 (1.10) |
3 | GTEKLTITY | No | 1.50 (0.90) |
SSSFSFGGF | No | 1.40 (0.80) | |
YVFVGTSRY | No | 1.50 (0.90) | |
ASYGAGAGY | Negative control | 1.20 (0.00) | |
CTELKLSDY | No | 6.30 (5.10) | |
4/ | GTEKLTITY | No | 6.50 (5.30) |
3 | SSSFSFGGF | No | 3.90 (2.70) |
YVFVGTSRY | No | 5.80 (4.60) | |
ASYGAGAGY | Negative control | 2.60 (0.00) | |
CTELKLSDY | Yes | 5.80 (3.20*) | |
6/ | GTEKLTITY | No | 5.80 (3.20) |
3 | SSSFSFGGF | No | 4.90 (2.30) |
YVFVGTSRY | Yes | 5.90 (3.30*) | |
ASYGAGAGY | Negative control | 0.90 (0.00) | |
CTELKLSDY | Yes | 3.00 (2.10*) | |
8/ | GTEKLTITY | No | 2.40 (1.50) |
4 | SSSFSFGGF | No | 1.90 (1.00) |
YVFVGTSRY | Yes | 2.70 (1.80*) | |
ASYGAGAGY | Negative control | 1.10 (0.00) | |
CTELKLSDY | Yes | 2.80 (1.70*) | |
16/ | GTEKLTITY | No | 2.50 (1.40) |
5 | SSSFSFGGF | No | 2.30 (1.20) |
YVFVGTSRY | Yes | 2.70 (1.60*) |