|
Animal ID/SwIV strain
|
Tetramer SwIV peptide
|
Peptide substituted from immunization strain
|
Frequency of tetramer (APC + BV421+) cells
|
|---|
|
(Tetramer + cells subtracted negative control)
|
|---|
| |
ASYGAGAGY
|
Negative control
|
0.80 (0.00)
|
|
1/
|
CTELKLSDY
|
No
|
1.70 (0.90)
|
|
1
|
GTEKLTITY
|
No
|
1.90 (1.10)
|
|
SSSFSFGGF
|
No
|
1.70 (0.90)
|
|
YVFVGTSRY
|
No
|
1.60 (0.80)
|
| |
ASYGAGAGY
|
Negative control
|
0.60 (0.00)
|
|
2/
|
CTELKLSDY
|
No
|
1.70 (1.10)
|
|
3
|
GTEKLTITY
|
No
|
1.50 (0.90)
|
|
SSSFSFGGF
|
No
|
1.40 (0.80)
|
|
YVFVGTSRY
|
No
|
1.50 (0.90)
|
| |
ASYGAGAGY
|
Negative control
|
1.20 (0.00)
|
|
CTELKLSDY
|
No
|
6.30 (5.10)
|
|
4/
|
GTEKLTITY
|
No
|
6.50 (5.30)
|
|
3
|
SSSFSFGGF
|
No
|
3.90 (2.70)
|
|
YVFVGTSRY
|
No
|
5.80 (4.60)
|
| |
ASYGAGAGY
|
Negative control
|
2.60 (0.00)
|
|
CTELKLSDY
|
Yes
|
5.80 (3.20*)
|
|
6/
|
GTEKLTITY
|
No
|
5.80 (3.20)
|
|
3
|
SSSFSFGGF
|
No
|
4.90 (2.30)
|
|
YVFVGTSRY
|
Yes
|
5.90 (3.30*)
|
| |
ASYGAGAGY
|
Negative control
|
0.90 (0.00)
|
|
CTELKLSDY
|
Yes
|
3.00 (2.10*)
|
|
8/
|
GTEKLTITY
|
No
|
2.40 (1.50)
|
|
4
|
SSSFSFGGF
|
No
|
1.90 (1.00)
|
|
YVFVGTSRY
|
Yes
|
2.70 (1.80*)
|
| |
ASYGAGAGY
|
Negative control
|
1.10 (0.00)
|
|
CTELKLSDY
|
Yes
|
2.80 (1.70*)
|
|
16/
|
GTEKLTITY
|
No
|
2.50 (1.40)
|
|
5
|
SSSFSFGGF
|
No
|
2.30 (1.20)
|
|
YVFVGTSRY
|
Yes
|
2.70 (1.60*)
|
- Tetramer staining frequencies. Percentile numbers in bold show specific tetramer staining post background subtraction. The relative background staining has been defined for each animal by a negative control tetramer (ASYGAGAGY). Italic percentile numbers indicate non-specific staining. Percentages marked by an asterix (*) indentify positive staining by influenza peptides which are sequence substituted compared to the respective immunization strains.
