Identification of swine influenza virus epitopes and analysis of multiple specificities expressed by cytotoxic T cell subsets

Table 3 Influenza virus tetramer staining

Animal ID/SwIV strain	Tetramer SwIV peptide	Peptide substituted from immunization strain	Frequency of tetramer (APC + BV421+) cells
Animal ID/SwIV strain	Tetramer SwIV peptide	Peptide substituted from immunization strain	(Tetramer + cells subtracted negative control)
	ASYGAGAGY	Negative control	0.80 (0.00)
1/	CTELKLSDY	No	1.70 (0.90)
1	GTEKLTITY	No	1.90 (1.10)
	SSSFSFGGF	No	1.70 (0.90)
	YVFVGTSRY	No	1.60 (0.80)
	ASYGAGAGY	Negative control	0.60 (0.00)
2/	CTELKLSDY	No	1.70 (1.10)
3	GTEKLTITY	No	1.50 (0.90)
	SSSFSFGGF	No	1.40 (0.80)
	YVFVGTSRY	No	1.50 (0.90)
	ASYGAGAGY	Negative control	1.20 (0.00)
	CTELKLSDY	No	6.30 (5.10)
4/	GTEKLTITY	No	6.50 (5.30)
3	SSSFSFGGF	No	3.90 (2.70)
3	YVFVGTSRY	No	5.80 (4.60)
	ASYGAGAGY	Negative control	2.60 (0.00)
	CTELKLSDY	Yes	5.80 (3.20*)
6/	GTEKLTITY	No	5.80 (3.20)
3	SSSFSFGGF	No	4.90 (2.30)
3	YVFVGTSRY	Yes	5.90 (3.30*)
	ASYGAGAGY	Negative control	0.90 (0.00)
	CTELKLSDY	Yes	3.00 (2.10*)
8/	GTEKLTITY	No	2.40 (1.50)
4	SSSFSFGGF	No	1.90 (1.00)
4	YVFVGTSRY	Yes	2.70 (1.80*)
	ASYGAGAGY	Negative control	1.10 (0.00)
	CTELKLSDY	Yes	2.80 (1.70*)
16/	GTEKLTITY	No	2.50 (1.40)
5	SSSFSFGGF	No	2.30 (1.20)
5	YVFVGTSRY	Yes	2.70 (1.60*)

Tetramer staining frequencies. Percentile numbers in bold show specific tetramer staining post background subtraction. The relative background staining has been defined for each animal by a negative control tetramer (ASYGAGAGY). Italic percentile numbers indicate non-specific staining. Percentages marked by an asterix (*) indentify positive staining by influenza peptides which are sequence substituted compared to the respective immunization strains.