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Table 3 Influenza virus tetramer staining

From: Identification of swine influenza virus epitopes and analysis of multiple specificities expressed by cytotoxic T cell subsets

Animal ID/SwIV strain Tetramer SwIV peptide Peptide substituted from immunization strain Frequency of tetramer (APC + BV421+) cells
(Tetramer + cells subtracted negative control)
  ASYGAGAGY Negative control 0.80 (0.00)
1/ CTELKLSDY No 1.70 (0.90)
1 GTEKLTITY No 1.90 (1.10)
SSSFSFGGF No 1.70 (0.90)
YVFVGTSRY No 1.60 (0.80)
  ASYGAGAGY Negative control 0.60 (0.00)
2/ CTELKLSDY No 1.70 (1.10)
3 GTEKLTITY No 1.50 (0.90)
SSSFSFGGF No 1.40 (0.80)
YVFVGTSRY No 1.50 (0.90)
  ASYGAGAGY Negative control 1.20 (0.00)
CTELKLSDY No 6.30 (5.10)
4/ GTEKLTITY No 6.50 (5.30)
3 SSSFSFGGF No 3.90 (2.70)
YVFVGTSRY No 5.80 (4.60)
  ASYGAGAGY Negative control 2.60 (0.00)
CTELKLSDY Yes 5.80 (3.20*)
6/ GTEKLTITY No 5.80 (3.20)
3 SSSFSFGGF No 4.90 (2.30)
YVFVGTSRY Yes 5.90 (3.30*)
  ASYGAGAGY Negative control 0.90 (0.00)
CTELKLSDY Yes 3.00 (2.10*)
8/ GTEKLTITY No 2.40 (1.50)
4 SSSFSFGGF No 1.90 (1.00)
YVFVGTSRY Yes 2.70 (1.80*)
  ASYGAGAGY Negative control 1.10 (0.00)
CTELKLSDY Yes 2.80 (1.70*)
16/ GTEKLTITY No 2.50 (1.40)
5 SSSFSFGGF No 2.30 (1.20)
YVFVGTSRY Yes 2.70 (1.60*)
  1. Tetramer staining frequencies. Percentile numbers in bold show specific tetramer staining post background subtraction. The relative background staining has been defined for each animal by a negative control tetramer (ASYGAGAGY). Italic percentile numbers indicate non-specific staining. Percentages marked by an asterix (*) indentify positive staining by influenza peptides which are sequence substituted compared to the respective immunization strains.