Figure 3

Blind clustering of scrapie-infected mice results in their grouping largely by time after infection. Hierarchical clustering of the scrapie-infected mice euthanized at 70, 90, 110, 130 dpi or at terminal stage of disease (TER) using the densitometric data of the 109 protein kinases detected in primary multiplex Western blots. The normalized and log₂ transformed levels of protein kinase expression for each set (1, 2, 3 - consisting of one scrapie-and one mock-infected mouse euthanized at each time point) were clustered using Gene Cluster 3.0 software (Euclidean distance; complete linkage) and presented using Java Treeview.