Figure 2

Differential expression of protein kinases in scrapie- versus mock-infected mice at 70 and 130 dpi. Primary multiplex Western blots analyzing the expression levels of 139 protein kinases in three mock- or scrapie-infected mice at 70 (A) or 130 (B) dpi. Mice identified by ID numbers; M, mock-infected; RML, RML-infected. Molecular weights in kDa are indicated on the left. The protein kinases CaMK4β (A; probed with antibody set 1) and DLK (B; probed with antibody set 2) are indicated by white arrowheads and enlarged to illustrate their differential expression in scrapie- versus mock-infected mice. The antibody used to detect CaMK4β also detects CaMK4 (red band, indicated by the asterisks), the levels of which remain constant. The expression levels of the other protein kinases tested in the same or adjacent lanes also remained constant or were expressed differently than CaMK4β and DLK (A, CK1ϵ [red band] indicated by 1; B, LIMK1 indicated by 1 and GSK3α indicated by 2). Apparent signal above GSK3α in mouse ID 11 M is an artifact.