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Figure 1 | Virology Journal

Figure 1

From: Activation of Pro-survival CaMK4β/CREB and Pro-death MST1 signaling at early and late times during a mouse model of prion disease

Figure 1

Levels of GFAP and PrPresduring scrapie progression. (A) Western blots of GFAP, total PrP, and PrPres in homogenates of brainstem-cerebellum, subcortical region, and cortical region of three mock (Mock)- or scrapie (RML)-infected mice at 70, 90, 110, 130 dpi or at terminal stage of disease (155–190 dpi). Molecular weights in kDa are indicated on the left. Black triangles, samples in which PrPres was quantitated. (B) Time-course changes in the levels of GFAP and PrPres. Line graphs show the levels of GFAP in scrapie-infected mice normalized to the average levels in mock-infected mice at each time point. The levels of PrPres are expressed as a percentage relative to the highest level detected in each region. Mean ± SD; n = 3 (Mean ± range for PrPres at 130 dpi where n = 2). Error bars on the x-axis, range in time of onset of terminal disease. The differences in GFAP levels in scrapie- versus mock-infected mice were analyzed by unpaired two-tail t-test. *P < 0.05; **P < 0.01; ***P < 0.001.

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