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Table 3 Primer sequences used for sequencing 3′ and 5′ NCRs of H3N8 EIV

From: An efficient genome sequencing method for equine influenza [H3N8] virus reveals a new polymorphism in the PA-X protein

Primer Primer sequence
Universal 3′ 5′-CCTTGTTTCTACTAGC-3′
Universal 5′ 5′-CCTGCTTTTGCTAGT-3′
PB2 3′ 5′-GG G TATTTCATTGCCATCATCC-3′
PB2 5′ 5′-GACTCTAGCATACTTACTGACAG-3′
PB1 3′ 5′-GAC A GT A TCCATGGTGTATCCTGT-3′
PB1 5′ 5′-G T ATGGT T GAGGCCATGGTGTC C-3′
PA 3′ 5′-A T C CCTGTGATTTCAAATCTTTCTTC-3′
PA 5′ 5′-GA A TGCCTGATTAATGATCCCTG-3′
HA 3′ 5′-A AT GTTCC A TTTG CT ACTGCATG-3′
HA 5′ 5′-GGATTTC A TT C GCCATATCATG-3′
NP 3′ 5′-TCAAA T GC C GA A AG T-3′
NP 5′ 5′-CCGATCGTGCC T TC C TTTGACA T-3′
NA 3′ 5′-GTGGAGTAGATCATAAAATTGCC-3′
NA 5′ 5′-CAGACCTGTTTCATTGTTATTGAG-3′
M 3′ 5′-A T AAAGCGTCTACGCTGCAGTCC-3′
M 5′ 5′-AAAGAGGGCCTTCTACGGAAGG-3′
NS 3′ 5′-C C GT A TTATCATTCCATT T AAG-3′
NS 5′ 5′-TGATAA T ACG GTTA GAA TCTCT-3′
  1. Sequences from de Wit et al., [20] shown in italics with specific individual nucleotide changes in bold text, plus novel primer sequences for the N8 NA segment.