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Figure 7 | Virology Journal

Figure 7

From: Nuclear localized Influenza nucleoprotein N-terminal deletion mutant is deficient in functional vRNP formation

Figure 7

del20NLS-NP does not form high molecular weight complexes. 293 T cells were transfected with plasmid mixes to express recombinant vRNPs containing influenza RdRP with either WT-NP, del20NLS-NP, or no NP, and GFP-NS1ss vRNA templates. Total protein extracts were separated on TBE (native) 6% PAGE facilitated by coomassie blue. Proteins were transferred to nitrocellulose and probed with anti-FLAG antibody to detect NP proteins and anti- NS1 antibody to detect NS1 proteins.

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