Figure 7From: Nuclear localized Influenza nucleoprotein N-terminal deletion mutant is deficient in functional vRNP formationdel20NLS-NP does not form high molecular weight complexes. 293Â T cells were transfected with plasmid mixes to express recombinant vRNPs containing influenza RdRP with either WT-NP, del20NLS-NP, or no NP, and GFP-NS1ss vRNA templates. Total protein extracts were separated on TBE (native) 6% PAGE facilitated by coomassie blue. Proteins were transferred to nitrocellulose and probed with anti-FLAG antibody to detect NP proteins and anti- NS1 antibody to detect NS1 proteins.Back to article page