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Figure 10 | Virology Journal

Figure 10

From: Nuclear localized Influenza nucleoprotein N-terminal deletion mutant is deficient in functional vRNP formation

Figure 10

GFP-M protein expression is not recovered by presence of NS1. 293 T cells were transfected with plasmid mixes to express recombinant vRNPs containing influenza RdRP with either WT-NP, del20NLS-NP, or no NP, and either GFP-M vRNA or FLAG-M vRNA in the presence or absence of NS1 as indicated. Total protein was separated by SDS-PAGE and analyzed by Western blotting. Anti-Udorn antibody detects NP and M1 products. Anti-NS1 detects NS1. Anti-tubulin detects tubulin and serves as a loading control. Shown is a representative experiment, repeated in triplicate.

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