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Figure 4 | Virology Journal

Figure 4

From: Influenza polymerase encoding mRNAs utilize atypical mRNA nuclear export

Figure 4

Nxf1 is associated with influenza NS1 protein in A549 cells. A549 cells were transfected with plasmid to express FLAG-Nxf1 or FLAG-Vector control and infected with influenza A Udorn or mock infected at 48 hours post transfection. Cells were collected 7 hours post infection and total extract was prepared and subject to immuno purification using anti-FLAG antibody coupled to beads. Proteins from the total extract (tot), immuno purified beads (IP), and supernatant (sup), were separated by SDS-PAGE and subject to Western blot with anti-FLAG antibody to detect FLAG-Nxf1 and anti-NS1 (Udorn) to detect influenza NS1 protein. Shown is a representative blot from one biological trial.

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