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Figure 7 | Virology Journal

Figure 7

From: Identification of molecular sub-networks associated with cell survival in a chronically SIVmac-infected human CD4+ T cell line

Figure 7

Caveolin-1 expression is induced in chronically infected C8166 T cells. A: Caveolin-1 mRNA expression is induced in human T cell chronically infected by SIV. Products from LightCycler PCR from mRNA isolated from infected and mock-infected T cells are shown. Specific exon-spanning primers were used to amplify Cav-1 or ribosomal protein 9 (RPS9) for normalization. Lanes 1, 2 acute infection; lanes 3, 4 chronically SIV-infected T cells; lanes 5, 6 mock-infected cells; lane 7, water control. The expected products of 318 bp (top Cav-1 lanes 3, 4) and 95 bp (bottom RPS9, lanes 1–6) were identified in 2% agarose gels. B: Western blot detection of caveolin-1 in SIVmac251 H32 C8166-P cells. Equal amounts of cell lysates from human T cell C8166 (lane 2) and from human T cell C8166 chronically infected by SIV (lane 3) were separated by SDS PAGE and Western blot was carried out by using rabbit anti-caveolin-1 antibody as the primary antibody. NIH 3T3 cell lysate (1/50 dilution) was used as the caveolin-1 positive control sample (lane1). Arrow depicts caveolin-1; in lane 1, the two isoforms of caveolin-1 found in NIH 3T3 cells are visible.

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