Skip to main content

Table 3 Mutations accrued in the C-prM-E genes of MODV-WNV(prM-E) during transfection and passage in designated cell types

From: Substitution of the premembrane and envelope protein genes of Modoc virus with the homologous sequences of West Nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells

Passage history

Nucleotide position

Amino acid position

Nucleotide change

Amino acid change

Original Inoculum (BSR-T7)

1457

E-167

C → T

Leu → Phe

BHK-21 + Vero + Vero

323

C-72

T → C

Silent

 

1457

E-167

C → T

Leu → Phe

 

1771

E-271

T → C

Silent

 

2372

E-472

A → G

Met → Val

Vero + Vero + Vero

462

prM-2

C → T

Thr → Ile

 

1307

E-117

G → A

Ala → Thr

 

1457

E-167

C → T

Leu → Phe

 

1894

E-216

T → C

Silent

 

2261

E-435

T → C

Phe → Leu

Back to article page

Virology Journal

ISSN: 1743-422X