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Table 3 Mutations accrued in the C-prM-E genes of MODV-WNV(prM-E) during transfection and passage in designated cell types

From: Substitution of the premembrane and envelope protein genes of Modoc virus with the homologous sequences of West Nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells

Passage history Nucleotide position Amino acid position Nucleotide change Amino acid change
Original Inoculum (BSR-T7) 1457 E-167 C → T Leu → Phe
BHK-21 + Vero + Vero 323 C-72 T → C Silent
  1457 E-167 C → T Leu → Phe
  1771 E-271 T → C Silent
  2372 E-472 A → G Met → Val
Vero + Vero + Vero 462 prM-2 C → T Thr → Ile
  1307 E-117 G → A Ala → Thr
  1457 E-167 C → T Leu → Phe
  1894 E-216 T → C Silent
  2261 E-435 T → C Phe → Leu