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Table 2 Primers used during the construction of full-length flavivirus chimeric DNAs

From: Substitution of the premembrane and envelope protein genes of Modoc virus with the homologous sequences of West Nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells

Primer

Polarity

Sequencea

Target

M-F1

Sense

5’ACATTTCCCCGAAAAGTGCCACCTGACGTCTCGAC3’

Cloning vector

MW-R1

Antisense

5’CCTCCTGGTTTCTTAGACAT TCCCGCCACAAAAAGTGG3’

WNV/MODV

MWi-R1

Antisense

5’TAACTGCTCCTACGCTGGCGAT TGACAATATGGTTCCCATCATCC3’

WNV/MODV

MC-R1

Antisense

5’CTTACCGTCGTCCTTTCCCAT TCCCGCCACAAAAAGTGG3’

CxFV/MODV

MCi-R1

Antisense

5’ACGGCGCCCAGCACCATCAT TGACAATATGGTTCCCATCATC3’

CxFV/MODV

MW-F2

Sense

5’CCACTTTTTGTGGCGGGAATGTCTAAGAAACCAGGAGG 3’

MODV/WNV

MWi-F2

Sense

5’ATGGATGATGGGAACCATATTGTCAATCGCCAGCGTAGGAGCAG 3’

MODV/WNV

MC-F2

Sense

5’CCACTTTTTGTGGCGGGAATGGGAAAGGACGACGGTAAG 3’

MODV/CxFV

MCi-F2

Sense

5’ATATGGATGATGGGAACCATATTGTCAATGATGGTGCTGGGCGCCGTC 3’

MODV/CxFV

MW-R2

Antisense

5’CAAGGACACAGCCATGATCAGCGTGCACGTTCACGGAG 3’

MODV/WNV

MC-R2

Antisense

5’CATCAAGGACACAGCCATGATCTGCCTTGGTGTAGATAAAGTATCC 3’

MODV/CxFV

MW-F3

Sense

5’CTCCGTGAACGTGCACGCT GATCATGGCTGTGTCCTTG3’

WNV/MODV

MC-F3

Sense

5’GGATACTTTATCTACACCAAGGCA GATCATGGCTGTGTCCTTGATG3’

CxFV/MODV

M-R3

Antisense

5’TCCATTTGCATTGATGACTGGAGAACCAGATGAACCAGGAGG3’

MODV

M-F4

Sense

5’AGACTCTTATTCTTGGGGTGGG3’

MODV

T7MOD-F

Sense

5’TAATACGACTCACTATAGG AGTTGATCCTGCCAGCGGTG3’

T7/MODV

M-R10600

Antisense

5’AGCGGAGGTCATATTCATGACCACACAGATTACATG3’

MODV

  1. aHeterologous virus sequences are italicized in chimeric primers, T7 promoter sequence is bolded.