Western blot analysis reveals the presence of WNV antigen in Vero cells, but not C6/36 cells, inoculated with MODV-WNV(prM-E). Lysates were prepared from (A) Vero and (B) C6/36 cells that had been mock-inoculated (lane 1) or inoculated with chimeric virus (lane 2), MODV (lane 3) or WNV (lane 4) at a m.o.i. of 0.1. Lysates were harvested at 7 days p.i. and equal amounts of protein were resolved on 8-16% Tris-glycine gels and immunoblotted using a pooled suspension of anti-WNV E protein monoclonal antibodies. M denotes the SDS PAGE low-range molecular weight standards (Invitrogen). The arrow shows the expected migration position of the WNV E protein (molecular weight: 53 KDa).