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Figure 2 | Virology Journal

Figure 2

From: Substitution of the premembrane and envelope protein genes of Modoc virus with the homologous sequences of West Nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells

Figure 2

Comparison of the plaque morphologies of MODV-WNV(prM-E) and the parental viruses in Vero cells. Confluent monolayers of Vero cells in six-well plates were inoculated with MODV-WNV(prM-E), MODV or WNV. Cells were fixed and plaques were visualized by staining with crystal violet at 3, 5 and 7 days p.i. Images were transferred into Microsoft Photoshop and plaque diameters were measured. The chimeric virus had been passaged one in BHK-21 cells and twice in Vero cells prior to this experiment.

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Virology Journal

ISSN: 1743-422X