Figure 2

Chromosomal distribution of integration junctions. (A) Summary of junction data obtained using both the left and right AAV-2 primer sets; the number in parenthesis represents biological replicates. (B) Unique integration events per mappable megabase of human chromosomes. (C) Genome-wide view of all integration events (red dots) and genes (blue bars). Darkness, size, and proximity to the center correspond with increasing insertions per locus. Chromosome sizes and banding patterns are presented in the outermost ring. (D) Profile of unique integrations around AAVS1 in 1 Kb intervals, with genes and gene orientation (blue arrow). RBS = Rep binding site of AAVS1 (red arrow). (E) Summary of most frequent AAV integration loci; all sites with greater than one insertion are shown.