Figure 1

Chitosan microparticles charged with yeast-derived porcine circovirus type 2 (PCV2) virus-like particles (VLPs) by antigen ionic gelation. Crude yeast extracts containing PCV2 VLPs were coated with low molecular weight chitosan. (A) Particle size and morphology by SEM. (B) The Zeta potential was measured to ascertain the average superficial charge (8.19 mV) and (C) the overall microparticle size and polydispersity index (0.005) was measured by light scatter analysis.