Table 4 Correlation of results from the novel system with degenerate probes, normal probes, the Roche diagnostic system, and from sequencing
From: Quantitation of substitutions at amino acid 70 in hepatitis C virus genotype 1b
No. | Roche system | Normal probes | Degenerate probes | Cloning sequencing | Matched with normal probes | Matched with degenerate probes | ||
|---|---|---|---|---|---|---|---|---|
RNA Load$ | Ratio* | Wild-type (%) | Ratio* | Wild-type (%) | Codon 70 | |||
1 | 7.2 | 0.19 | 82.1 | 1.33 | 98.5 | 4CGG/1CAG/14CGA/1 N.D.# | 4CGG/1CAG | 18CGN/1CAN |
2 | 6.3 | 0.01 | 0.0 | 1.27 | 2.6 | 13CAA/7CAT | None | 20CAN |
3 | 7.0 | 0.08 | 4.2 | 1.38 | 92.0 | 2CAG/18CGA | 2CAG | 2CAN/18CGN |
4 | 6.6 | 0.07 | 100.0 | 0.71 | 97.5 | 15CGA/5 N.D. | None | 15CGN |
5 | 6.6 | 0.08 | 100.0 | 0.87 | 100.0 | 1CGG/19CGT | 1CGG | 20CGN |
6 | 5.6 | 0.17 | 4.8 | 1.39 | 2.7 | 3CAG/17CAA | 3CAG | 20CAN |
7 | 6.5 | 0.02 | 63.2 | 0.70 | 92.7 | 2CAA/16CGA/2 N.D. | None | 2CAN/16CGN |
8 | 7.9 | 0.04 | 0.0 | 0.80 | 0.0 | 18CAA/2 N.D. | None | 18CAN |
9 | 7.1 | 0.03 | 2.0 | 1.48 | 1.5 | 19CAC/1 N.D. | None | 19CAN |
10 | 6.4 | 0.13 | 20.6 | 1.47 | 4.2 | 1CGG/3CAG/16CAA | 1CGG/3CAG | 1CGN/19CAN |
11 | 5.6 | 0.12 | 95.6 | 1.18 | 96.4 | 3CGG/17CGA | 3CGG | 20CGN |
12 | 7.4 | 0.08 | 96.0 | 1.32 | 8.0 | 1CGG/15CAT/4 N.D. | 1CGG | 1CGN/15CAN |
13 | 6.7 | 0.08 | 91.3 | 0.96 | 9.8 | 2CGG/16CAA/2 N.D. | 2CGG | 2CGN/16CAN |
14 | 6.3 | 0.18 | 95.5 | 1.90 | 97.5 | 4CGG/16CGA | 4CGG | 20CGN |