Quantitation of substitutions at amino acid 70 in hepatitis C virus genotype 1b

Table 1 Detection limits when using the plasmid mixture as templates

Probes	W:M plasmid ratio	Plasmid concentration (copies/μL)
		10¹	10²	10³	10⁴	10⁵	10⁶	10⁷
Wild-type	99:1	D	D	D	D	D	D	D
	90:10	D	D	D	D	D	D	D
	80:20	D	D	D	D	D	D	D
	50:50	D	D	D	D	D	D	D
	20:80	D	D	D	D	D	D	D
	10:90	D	D	D	D	D	D	D
	1:99	U	D	D	D	D	D	D
Mutant	99:1	U	D	D	D	D	D	D
	90:10	D	D	D	D	D	D	D
	80:20	D	D	D	D	D	D	D
	50:50	D	D	D	D	D	D	D
	20:80	D	D	D	D	D	D	D
	10:90	D	D	D	D	D	D	D
	1:99	D	D	D	D	D	D	D

ISSN: 1743-422X