|
Primers
|
Sequences (5′-3′)
|
Position
|
Expected extent (bp)
|
Expected size (bp)
|
|---|
|
1Fa
|
ACCAAACAGAGAATCCGTGAG
|
1-21
|
1-1 270
|
1 270
|
|
1Rb
|
CTTTAGCTCGGCAGCCATATCCTC
|
1258-1270
|
|
2F
|
TGAGCACATCATTCTGGAGACTTG
|
1185-1207
|
1 185–2 280
|
1 096
|
|
2R
|
TGGACGATTTATTGCTAAGCTTG
|
2259-2280
|
|
3F
|
CTAAGCACAGCATGGGAGAAG
|
2025-2046
|
2 025–3 432
|
1 408
|
|
3R
|
AAGTCAAGACGCTGGATCCT
|
3413-3432
|
|
4F
|
CACAGGAGATGGGAAGAAGC
|
3376-3395
|
3 376–5 330
|
1 955
|
|
4R
|
ATGAGCTGAGTTGATTGTTCC
|
5310-5330
|
|
5F
|
CAGTTGGGAAGATGCAGCAG
|
5076-5095
|
5 076–6 873
|
1 798
|
|
5R
|
AGAGGGATAGAATGATGTGAC
|
6853-6873
|
|
6F
|
CCTGTTCATGACCCAGACTAC
|
6787-6807
|
6 787–8 520
|
1 734
|
|
6R
|
TCGAAGTCACATTCATCAGG
|
8501-8520
|
|
7F
|
GATCAGATGAGAGCCACTACAA
|
6471-6492
|
6 471–8 448
|
1 978
|
|
7R
|
GATAGATGTGACTCTGGTAGGAT
|
8426-8448
|
|
8F
|
AGAGGGAACACGGGTAGGA
|
8376-8394
|
8 367–10 054
|
1 688
|
|
8R
|
CTTAGCAAAAATCCGCCCA
|
10036-10054
|
|
9F
|
ATCGTACTCACTCAAAGAGA
|
10000-10019
|
10 000–11 747
|
1 748
|
|
9R
|
GGGCATAATCTGCTAGCGTC
|
11728-11747
|
|
10F
|
TGACGCTAGCAGATTATG
|
11727-11744
|
11 727–13 422
|
1 696
|
|
10R
|
TCCTGAGGGAACAACAAT
|
13405-13422
|
|
11F
|
TCTTTCCAATGACAACAACC
|
12604-12623
|
12 604–14 589
|
1 986
|
|
11R
|
GACCGGATAACTGTGTCAAT
|
14570-14589
|
|
12F
|
AAGATTCGTCCAGGGTCC
|
14033-14050
|
14 033–15 192
|
1 160
|
|
12R
|
ACCAAACAGAGATTTGGTGAA
|
15172-15192
|
- aF stands for forward primer.
- bR stands for reverse primer.
