Table 9 Primers used in the study
Primers | Sequences (5′-3′) | Position | Expected extent (bp) | Expected size (bp) |
|---|---|---|---|---|
1Fa | ACCAAACAGAGAATCCGTGAG | 1-21 | 1-1 270 | 1 270 |
1Rb | CTTTAGCTCGGCAGCCATATCCTC | 1258-1270 | ||
2F | TGAGCACATCATTCTGGAGACTTG | 1185-1207 | 1 185–2 280 | 1 096 |
2R | TGGACGATTTATTGCTAAGCTTG | 2259-2280 | ||
3F | CTAAGCACAGCATGGGAGAAG | 2025-2046 | 2 025–3 432 | 1 408 |
3R | AAGTCAAGACGCTGGATCCT | 3413-3432 | ||
4F | CACAGGAGATGGGAAGAAGC | 3376-3395 | 3 376–5 330 | 1 955 |
4R | ATGAGCTGAGTTGATTGTTCC | 5310-5330 | ||
5F | CAGTTGGGAAGATGCAGCAG | 5076-5095 | 5 076–6 873 | 1 798 |
5R | AGAGGGATAGAATGATGTGAC | 6853-6873 | ||
6F | CCTGTTCATGACCCAGACTAC | 6787-6807 | 6 787–8 520 | 1 734 |
6R | TCGAAGTCACATTCATCAGG | 8501-8520 | ||
7F | GATCAGATGAGAGCCACTACAA | 6471-6492 | 6 471–8 448 | 1 978 |
7R | GATAGATGTGACTCTGGTAGGAT | 8426-8448 | ||
8F | AGAGGGAACACGGGTAGGA | 8376-8394 | 8 367–10 054 | 1 688 |
8R | CTTAGCAAAAATCCGCCCA | 10036-10054 | ||
9F | ATCGTACTCACTCAAAGAGA | 10000-10019 | 10 000–11 747 | 1 748 |
9R | GGGCATAATCTGCTAGCGTC | 11728-11747 | ||
10F | TGACGCTAGCAGATTATG | 11727-11744 | 11 727–13 422 | 1 696 |
10R | TCCTGAGGGAACAACAAT | 13405-13422 | ||
11F | TCTTTCCAATGACAACAACC | 12604-12623 | 12 604–14 589 | 1 986 |
11R | GACCGGATAACTGTGTCAAT | 14570-14589 | ||
12F | AAGATTCGTCCAGGGTCC | 14033-14050 | 14 033–15 192 | 1 160 |
12R | ACCAAACAGAGATTTGGTGAA | 15172-15192 |