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Table 9 Primers used in the study
| Primers | Sequences (5′-3′) | Position | Expected extent (bp) | Expected size (bp) |
|---|---|---|---|---|
| 1Fa | ACCAAACAGAGAATCCGTGAG | 1-21 | 1-1 270 | 1 270 |
| 1Rb | CTTTAGCTCGGCAGCCATATCCTC | 1258-1270 | ||
| 2F | TGAGCACATCATTCTGGAGACTTG | 1185-1207 | 1 185–2 280 | 1 096 |
| 2R | TGGACGATTTATTGCTAAGCTTG | 2259-2280 | ||
| 3F | CTAAGCACAGCATGGGAGAAG | 2025-2046 | 2 025–3 432 | 1 408 |
| 3R | AAGTCAAGACGCTGGATCCT | 3413-3432 | ||
| 4F | CACAGGAGATGGGAAGAAGC | 3376-3395 | 3 376–5 330 | 1 955 |
| 4R | ATGAGCTGAGTTGATTGTTCC | 5310-5330 | ||
| 5F | CAGTTGGGAAGATGCAGCAG | 5076-5095 | 5 076–6 873 | 1 798 |
| 5R | AGAGGGATAGAATGATGTGAC | 6853-6873 | ||
| 6F | CCTGTTCATGACCCAGACTAC | 6787-6807 | 6 787–8 520 | 1 734 |
| 6R | TCGAAGTCACATTCATCAGG | 8501-8520 | ||
| 7F | GATCAGATGAGAGCCACTACAA | 6471-6492 | 6 471–8 448 | 1 978 |
| 7R | GATAGATGTGACTCTGGTAGGAT | 8426-8448 | ||
| 8F | AGAGGGAACACGGGTAGGA | 8376-8394 | 8 367–10 054 | 1 688 |
| 8R | CTTAGCAAAAATCCGCCCA | 10036-10054 | ||
| 9F | ATCGTACTCACTCAAAGAGA | 10000-10019 | 10 000–11 747 | 1 748 |
| 9R | GGGCATAATCTGCTAGCGTC | 11728-11747 | ||
| 10F | TGACGCTAGCAGATTATG | 11727-11744 | 11 727–13 422 | 1 696 |
| 10R | TCCTGAGGGAACAACAAT | 13405-13422 | ||
| 11F | TCTTTCCAATGACAACAACC | 12604-12623 | 12 604–14 589 | 1 986 |
| 11R | GACCGGATAACTGTGTCAAT | 14570-14589 | ||
| 12F | AAGATTCGTCCAGGGTCC | 14033-14050 | 14 033–15 192 | 1 160 |
| 12R | ACCAAACAGAGATTTGGTGAA | 15172-15192 |
