Phylogenetic relationships and pathogenicity variation of two Newcastle disease viruses isolated from domestic ducks in Southern China

Table 3 Replication in chickens of NDV after inoculated intranasally ^a

Virus replication on 3 DPI (log₁₀EID₅₀/g)^bin:	Strains
Virus replication on 3 DPI (log₁₀EID₅₀/g)^bin:	SS-10	NH-10
Heart	4.56 ± 0.88	4.58 ± 0.58
Liver	5.5 ± 0.25	5.42 ± 0.14
Spleen	7.42 ± 0.14	7.08 ± 0.29
Lung	8.33 ± 0.14	7.5 ± 0.25
Kidney	6.17 ± 0.38	5.83 ± 0.63
Brain	5.08 ± 0.58	4.5 ± 0.91
Trachea	6.08 ± 0.29	5.08 ± 0.76
Small intestine	7.33 ± 0.14	6.83 ± 0.63
Bursa of fabricius	7.33 ± 0.14	7.42 ± 0.14
Thymus	7.5	7.17 ± 0.38
Pancreas	4.83 ± 1.38	5.75 ± 0.5
Cecal tonsils	7.5 ± 0.25	6.67 ± 0.52

^aSix-week-old chickens were inoculated intranasally (i.n.) with 10⁶ EID₅₀ of each virus in a 0.2 ml volume; three chickens in each group were euthanized on 3 DPI, and virus titer was determined in samples of heart, liver, spleen, lung, kidney, brain, trachea, Small intestine, bursa of fabricius, Thymus, Pancreas and cecal tonsils in eggs [17].
^bFor statistical analysis, a value of 1.5 was assigned if the virus was not detected from the undiluted sample in three embryonated hen eggs. Virus titers are expressed as means ± standard deviation in log₁₀EID₅₀/g of tissue.