Virus replication on 3 DPI (log10EID50/g)bin:
|
Strains
|
---|
SS-10
|
NH-10
|
---|
Heart
|
4.56 ± 0.88
|
4.58 ± 0.58
|
Liver
|
5.5 ± 0.25
|
5.42 ± 0.14
|
Spleen
|
7.42 ± 0.14
|
7.08 ± 0.29
|
Lung
|
8.33 ± 0.14
|
7.5 ± 0.25
|
Kidney
|
6.17 ± 0.38
|
5.83 ± 0.63
|
Brain
|
5.08 ± 0.58
|
4.5 ± 0.91
|
Trachea
|
6.08 ± 0.29
|
5.08 ± 0.76
|
Small intestine
|
7.33 ± 0.14
|
6.83 ± 0.63
|
Bursa of fabricius
|
7.33 ± 0.14
|
7.42 ± 0.14
|
Thymus
|
7.5
|
7.17 ± 0.38
|
Pancreas
|
4.83 ± 1.38
|
5.75 ± 0.5
|
Cecal tonsils
|
7.5 ± 0.25
|
6.67 ± 0.52
|
- aSix-week-old chickens were inoculated intranasally (i.n.) with 106 EID50 of each virus in a 0.2 ml volume; three chickens in each group were euthanized on 3 DPI, and virus titer was determined in samples of heart, liver, spleen, lung, kidney, brain, trachea, Small intestine, bursa of fabricius, Thymus, Pancreas and cecal tonsils in eggs [17].
- bFor statistical analysis, a value of 1.5 was assigned if the virus was not detected from the undiluted sample in three embryonated hen eggs. Virus titers are expressed as means ± standard deviation in log10EID50/g of tissue.