Figure 5

Absence of Tregs enhances adaptive immune response of CD4⁺and CD8⁺T cells. (A) Proportion of CD69⁺ among CD8⁺ and (B) CD4⁺ T cells 40 h p.i. (C) Percentage of CD3⁺ cells among live splenocytes on day 7 p.i. stratified by CD8 and CD4 expression. (D) CD62L^low cells within the CD8⁺ and (E) CD4⁺ T cell compartment as well as (F) KLRG-1 expression of CD8⁺ T cells 7 days p.i. (G) Quantification of IFN-γ⁺ cells among CD8⁺ T cells and (H) CD4⁺ T cells upon ex vivo stimulation with PMA / Ionomycin obtained from spleens of mice infected for 7 days. Data shown are from one representative experiment out of three for (A), (B), (C), (D), (E) and out of two (F), (G) and (H) using 3-5 mice per group. Significance of differences between means of groups was calculated by two tailed, unpaired Student’s t-test. (*) p < 0,05 (**) p < 0,01, (***) p < 0,001, (ns) not significantly different.