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Figure 1 | Virology Journal

Figure 1

From: Mouse Cytomegalovirus infection overrules T regulatory cell suppression on natural killer cells

Figure 1

MCMV infection elevates Treg proportion in CD4+T cell compartment early upon infection and DT administration results in efficient depletion of Tregs in DEREG mice. (A) Infection and depletion scheme of experimental procedure. (B) FoxP3+ cells among splenic CD4+CD3+ cells 40 h p.i. (C) the proportion of CD69+ cells among them and (D) their mean fluorescence intensity (MFI) of CD25 expression. (E) shows the percentage of FoxP3+ cells in the CD4+ T cell compartment on day 3 p.i., (F) indicates the CD69+ cells within this subset and (G) the MFI of CD25 expression. (H) The frequency of FoxP3+ cells among CD4+ T cells on day 7 p.i. (I) shows the CD25 expression on FoxP3+ cells and (J) the MFI of CTLA-4 expression on FoxP3+ cells on day 7 p.i. Data shown are from one representative experiment out of three in case of frequency analysis (B), (E) and (H) and out of at least two with regard to activation markers (C), (D), (F), (G), (I) and (J) using 3-5 mice per group. Significance of differences between means of groups was calculated by two tailed, unpaired Student’s t-test. (**) p < 0,01, (***) p < 0,001, (ns) not significantly different.

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