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Table 2 Sequences of primers used to amplify the 3,408 fragment of ball python Nidovirus

From: Identification of a novel nidovirus in an outbreak of fatal respiratory disease in ball pythons (Python regius)

Primer name*

Sequence 5′ – 3′

Nucleotide position

BPNV 1Fwd BPNV 1Rev

ACCTGCTACCGATGTCCAAG GTCGTTGTTGGCTGAGTGTG

1-963

BPNV 2Fwd BPNV 2Rev

TTCAAGCGAACCAAGTTCATCC TCTTGGACATCGGTAGCAGG

718-1485

BPNV 3Fwd BPNV 3Rev

AACATCCTCGACAACGCAGG ACGTAGTCTTGCCAGTTCCC

1446-1647

BPNV 4Fwd BPNV 4Rev

CCACAACCCGACAGTCAGTA GTACGTAGTCTTGCCAGTTCC

1456-2154

BPNV 5Fwd BPNV 5Rev

GGCACAGTAACAGCACAACG GTACTGCAAGATGCCGTTGC

2152-3030

BPNV 6Fwd BPNV 6Rev

GTGACTACACGAAATGCGACC GTCAAACATGAAAGCGTGCG

2995-3131

BPNV 7Fwd BPNV 7Rev

GTCGTCAACTTGTCCCACCA CTGCCATGCTACGGAAGACT

3088-3408

  1. *PCR cycling conditions: 95°C for 10 minutes followed by 35 cycles of 95° C for 30 seconds, 55°C for 30 seconds, 72°C for 1 minute, except primer pair BPNV 1 and BPNV 6 where annealing temperature was 53°C.