Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus

Table 3 Primers for amplification of RSV gene fragment

Primer name	Primer sequence (5’-3’)	Gene products (bp)
pF RSV-A	ACGCGTCGAC ACGCGAAAAAATGCGTACAACAAAC	2130 bp
pR RSV-A	AGCCCTAGG CCAGCAGCATTGCCTAATACTAC
pF RSV-B	AGCCCTAGG CATAATGGGAGAGTACAGAGG	2323 bp
pR RSV-B	CCGCTCGAG CTCTTGGTAACTCAAAGGTTTTG
pF RSV-C	CCGCTCGAG TCAACACATAGCATTCATCAATC	1200 bp
pR RSV-C	GCGGCCGC TTGTGATAATATGTTTTTTAATAAC
pF RSV-D	GCGGCCGC GACCAACTCAAACAGAATCAAAAT	2800 bp
pR RSV-D	CGCGGATCC ATTTTGTCCCACAGCTTGAATTAT
pF RSV-E	CGCGGATCC CATTATTAATGGAAATTCTGCTAA	2502 bp
pR RSV-E	CTAGCTAGC AAATAATCTGCTTGAGCATGAGT
pF RSV-F	CTAGCTAGC ATTAAATAGCCTTAAATTACTGTA	4218 bp
pR RSV-F	CGGGGTACC ACGAGAAAAAAAGTGTCAAAAACT

All the primers were designed based on the whole sequence of long strain of Respiratory syncytial virus (GenBank Accession: AY911262.1). The restriction enzyme sites within the primers are italic. “F” stands for forward and “R” stands for reverse to represent the direction of primer extension.

ISSN: 1743-422X