Skip to main content

Table 2 Titers of RSV in the upper and lower respiratory tract of BALB/c mice

From: Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus

  Lung Nose
Immunogena Virus Days postinfectionb Mean RSV titer ± SDdlog PFU ml−1 Mice protectedc(%) Mean RSV titer ± SDdlog PFU ml−1 Mice protectedc(%)
FI-RSV RSV-Long 3 4.56 ± 0.28 0 4.98 ± 0.38 0
5 5.11 ± 0.45 0 5.28 ± 0.55 0
7 4.78 ± 0.36 0 5.08 ± 0.25 0
10 4.96 ± 0.20 0 4.85 ± 0.48 0
21 3.38 ± 0.18 0 3.55 ± 0.33 0
rLong RSV-Long 3 <2.68 100 2.58 ± 0.28 75
5 <2.68 100 2.96 ± 0.20 75
7 <2.68 100 <1.98 100
10 <2.68 100 <1.98 100
21 <2.68 100 <1.98 100
rLong-∆G-EGFP RSV-Long 3 <2.68 100 2.69 ± 0.24 75
5 <2.68 100 3.17 ± 0.16 50
7 <2.68 100 2.25 ± 0.21 75
10 <2.68 100 <1.98 100
21 <2.68 100 <1.98 100
Mock RSV-Long 7 4.18 ± 0.25 0 4.03 ± 0.20 0
Mock Mock 7 <2.68 100 <1.98 100
  1. aBALB/c mice were infected with 106 PFU (100 μl) of the indicated virus (RSV-Long) at day 0. bLungs and nasal washes were harvested, and virus titers were determined at the indicated times. cResidual titers of RSV in the lung and nasal washes at the indicated time points post-challenge; protective efficacy of animals for the indicated groups of mice (FI-RSV, rLong, rLong-ΔG-EGFP). dThe lower limits of detection for virus in the lungs and nose were 2.68 log10 PFU g−1 and 1.98 log10 PFU ml−1, respectively. Groups at indicated time point consisted of four animals.