Identification of recovered recombinant viruses by PCR. (A) Amplification patterns of G gene for recovered viruses. lane 1, 2, RT-PCR product of RSV long strain and the recovered rLong (length 897 bp); lane 3, PCR product without band for rLong-∆G-EGFP. (B) identification of EGFP gene for recovered viruses. lane 1, 2, PCR product without any amplification band, lane 3, The RT-PCR products of EGFP gene (length 750 bp) for rLong-∆G-EGFP was electrophoresed through a 1% agarose gel.