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Figure 12 | Virology Journal

Figure 12

From: Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus

Figure 12

Recovery of infectious recombinant RSV long virus entirely from cloned cDNAs. The five cDNAs are indicated by rectangles and encode a positive-sense copy of the viral genome (antigenome) of rLong or rLong-∆G-EGFP and mRNAs for the N, P, L, and M2-1 proteins of the nucleocapsid/polymerase complex. The expression of the cDNAs is under the control of T7 promoter, The indicated ribozyme encoded at the downstream end of the antigenome executes self-cleavage to produce a correct 3’end. The T7 RNA polymerase can be provided intracellularly by vTF7-3 vaccinia virus.

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Virology Journal

ISSN: 1743-422X