First genome sequences of Achromobacter phages reveal new members of the N4 family

Table 1 Comparison of homologous structural genes of different N4-like phages

Identified proteins of JWAlpha	Homologous genes in Erwinia phage vB_EamP-S6	Homologous genes in Pseudomonas phage LIT1	Homologous genes in Vibrio phage φJA1
vRNAP, 369 kDa	gp79, 380.6 kDa, 28% aa identities	gp71, 369.7 kDa, 23% aa identities	gp53, 335 kDa, 23% aa identities
Structural protein, gp69 66.2 kDa	gp80, 55.9 kDa, no similarities at the aa level	gp72, 52.5 kDa, no similarities at the aa level	gp54, 47.1 kDa, no similarities at the aa level, not identified
Structural protein, gp70, 17.6 kDa	gp81, 16.3 kDa, 40% aa identities	gp73, 16.6 kDa, 38% aa identities	gp55, 15.3 kDa, 31% aa identities, not identified
Major coat, 44.4 kDa	gp85, 43.7 kDa, 66% aa identities	gp77, 44 kDa, 53% aa identities	gp59, 47 kDa, 44% aa identities
Portal, 85.4 kDa	gp88, 81.3 kDa, 60% aa identities	gp80, 81.7 kDa, 48% aa identities	gp62, 79.2 kDa, 47% aa identities
Tail component, gp82, 87.8 kDa			gp65, 87.3 kDa, no similarities at the aa level, not identified
Tail component, gp83, 26.3 kDa	gp97, 26.7 kDa, 36% aa identities, not identified	gp83, 27.9 kDa, 26% aa identities	gp66, 25.8 kDa, 32% aa identities, not identified
Surface protein, gp20, 33.8 kDa

Orf, molecular mass and similarities to JWAlpha at the amino acid level are shown. If not identified by peptide mass fingerprinting, proposed homologs in the phage genomes are listed.