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Table 2 Sensitivity of the RT-LAMP assay

From: Detection of Middle East respiratory syndrome coronavirus using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Copies/reaction

500,000

50,000

5000

500

50

5

0.5

Negative control

Sensitivity (copies)

Real-time RT-PCR*

       

upE

21.2

24.4

27.9

31.3

34.2

36.6

> 40

> 40

1.6

ORF1a

21.6

24.4

27.6

30.4

32

31.8

> 40

> 40

3.4

RT-LAMP**

11.33

12.13

13.07

15.44

21.44

22.33

> 30

> 30

3.4

  1. *Threshold cycle.
  2. **Time (min. s).