Table 4 Operational analysis of manual and automated extraction procedures
From: Development and validation of rapid magnetic particle based extraction protocols
Operator step | Required operator time (minutes) | |||
|---|---|---|---|---|
Manual | EZ1 | BS 15 | KF Duo | |
Filling of reagents | 5a | 15 | 5 | 5 |
Mix sample + lysis buffer | 10 | 5 | 10 | 10 |
Incubation | 10 | 0 | 0 | 0 |
Load instrument | 0 | 5 | 1 | 1 |
Extraction/Instrument run | 30 | 16 | 7.5 | 8 |
Total hands-on time | 40 - 45 | 25 | 16 | 16 |
Total processing time | 50 - 55 | 41 | 24 | 24 |