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Table 4 Operational analysis of manual and automated extraction procedures

From: Development and validation of rapid magnetic particle based extraction protocols

Operator step Required operator time (minutes)
Manual EZ1 BS 15 KF Duo
Filling of reagents 5a 15 5 5
Mix sample + lysis buffer 10 5 10 10
Incubation 10 0 0 0
Load instrument 0 5 1 1
Extraction/Instrument run 30 16 7.5 8
Total hands-on time 40 - 45 25 16 16
Total processing time 50 - 55 41 24 24
  1. adissolving carrier RNA.
  2. The time required for each operator step was measured for the extraction of 8 samples, respectively.