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Table 2 Reproducibility of manual and rapid automated extraction techniques

From: Development and validation of rapid magnetic particle based extraction protocols

Extraction Mean Cqvalue Intra-run Inter-run
SD CV% SD CV%
Manual 23.83 0.19 0.80 0.14 0.58
EZ1 24.10 0.16 0.65 0.12 0.51
BS 15 25.12 0.34 1.36 0.28 1.10
KF Duo 25.31 0.25 1.00 0.20 0.79
  1. SD = standard deviation, CV = coefficient of variation.
  2. Mean Cq values, standard deviations of replicates as well as intra- and inter-assay coefficients of variation were defined using 6 replicates of an SBV-positive blood sample in 3 independent runs.