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Table 2 Reproducibility of manual and rapid automated extraction techniques

From: Development and validation of rapid magnetic particle based extraction protocols

Extraction

Mean Cqvalue

Intra-run

Inter-run

SD

CV%

SD

CV%

Manual

23.83

0.19

0.80

0.14

0.58

EZ1

24.10

0.16

0.65

0.12

0.51

BS 15

25.12

0.34

1.36

0.28

1.10

KF Duo

25.31

0.25

1.00

0.20

0.79

  1. SD = standard deviation, CV = coefficient of variation.
  2. Mean Cq values, standard deviations of replicates as well as intra- and inter-assay coefficients of variation were defined using 6 replicates of an SBV-positive blood sample in 3 independent runs.
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Virology Journal

ISSN: 1743-422X