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Table 1 Speed-optimized extraction protocols for the KF Duo and the BS 15 instrument

From: Development and validation of rapid magnetic particle based extraction protocols

Step Reagents Timing of protocol steps (min)
Original KF Duo BS 15
Binding VXL + ACB + Beads 5 2 2
Wash 1 AW1 3 12 s 1
Wash 2 AW2 2 12 s 1
Wash 3 Ethanol 100% 2 12 s -
Evaporation Water 5 0 0
Elution AVE 2 1 0.5
Total time   24:00 8:00 7:50
  1. Each step of the original protocol was shortened to the maximum for the respective extraction platform.