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Figure 3 | Virology Journal

Figure 3

From: Effects of two amino acid substitutions in the capsid proteins on the interaction of two cell-adapted PanAsia-1 strains of foot-and-mouth disease virus serotype O with heparan sulfate receptor

Figure 3

Effects of heparin and the RGD-containing peptide on the plaque formation by the specific FMDVs on WT-CHO cells and BHK-21 cells. The plaque reduction neutralization assay was performed on two different cell types and the plaque inhibition assay was performed on BHK-21 cells, following the procedures as described in Methods. n = the number of 100% PFU (10–50 plaques/well) formed by the appropriate dilutions of the indicated FMDVs. (A) Heparin treatment greatly reduced production of plaques with four HS-utilizing viruses on WT-CHO cells. (B) The numbers of plaques of rHN, rHN/FJ9-VP0 and rHN/TAR6-VP0Q2080L were significantly reduced on BHK-21 cells, by addition of heparin; and (C) pre-incubation of the RGD-containing peptide VR-17 inhibited the plaque formation of O/Tibet/CHA/6/99tc on BHK-21 cells, despite the distinct plaque phenotypes of these four viruses on WT-CHO cells.

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Virology Journal

ISSN: 1743-422X