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Figure 8 | Virology Journal

Figure 8

From: Mutations within the conserved NS1 nuclear export signal lead to inhibition of influenza A virus replication

Figure 8

Effect of leptomycin B treatment on NS1 intracellular localization. A549 cells were infected with wild-type A/Udorn/72 virus and incubated with or without 2 ng/ml LMB added at 3 h postinfection. 20 h postinfection the cells were fixed and the intracellular localization of NS1 protein and NP were visualized by immunofluorescence and estimated in a quantitative manner by observing 6–8 random frames under the microscope. The pictures were taken by Leica TCS NT confocal microscope. Data shown is representative of three independent experiments.

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