Figure 4

Growth kinetics of the recombinant viruses in MDCK cells. MDCK cells on 6-well plates were infected at MOI values of 0.001 for 1 h after which virus inoculum was removed and fresh growth medium was added. The cells were incubated at 37°C humidified 5% CO₂ conditions and supernatant samples were taken at 12 h intervals and analyzed by endpoint dilution on MDCK cells. Mean values from three independent experiments are shown for each sample.