Figure 3

Localization patterns of wild-type and mutant NS1 proteins in A549 cells. A549 cells were infected with different recombinant A/Udorn/72 viruses for times indicated after which they were fixed, permeabilized and processed for detection by immunofluorescence. For each time point five random frames under the fluorescence microscope (≈100 cells) were inspected and the intracellular localization of NS1 protein was estimated and presented in a quantitative manner. Data shown is from the same samples as the immunofluorescence pictures in Figure 2.