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Figure 2 | Virology Journal

Figure 2

From: Mutations within the conserved NS1 nuclear export signal lead to inhibition of influenza A virus replication

Figure 2

Nuclear-cytoplasmic localization of wild-type and mutant NS1 proteins in A549 cells during infection. A549 cells were infected with the recombinant A/Udorn/72 viruses at MOI 1 for times indicated in the figure followed by fixation with 3% paraformaldehyde. Cells were permeabilized, stained with guinea pig anti-NS1 and secondary antibodies and processed for detection by immunofluorescence microscopy. The pictures were taken with Leica TCS NT confocal microscope. Data shown is representative of three independent experiments.

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Virology Journal

ISSN: 1743-422X