Figure 1

JUNV productive infection of NHA leads to amplification with time of immune/inflammatory response. A. JUNV production in NHA. Cells were infected with the virus at indicated MOIs and supernatants were harvested daily. Production of infectious virus was measured by plaque assay. B, C. Heat map plot of differentially expressed Immune/Inflammatory (B) and IFN (C) signaling transcripts for 24 and 96 h p.i. Rows are arranged by hierarchical clustering following Z-score transformation of the normalized data. Columns represent mock-infected (M24 and M96) and JUNV-infected (J24 and J96) tissue culture triplicates at 24 and 96 h p.i., respectively. Blue and green notches represent differentially expressed IFN signaling transcripts for 24 and 96 h time points p.i., respectively. D. Volcano plots of microarray results with identification of differentially expressed IFN-stimulated genes transcripts (red circles) for 24 (Di) and 96 (Dii) h p.i. The horizontal line represents significance (P < 0.0005) and the vertical lines represent the change in gene expression of JUNV-infected NHA compared with that of Mock-infected cells [Log2(fold change) > 1].