HSV-1 shedding from HaCat- and HMK cell lines after HSV-1 infection at 5 MOI. HSV-1 shedding into medium is measured using plaque titration assays on Vero cells. The data is derived from quadruplicate cultures measured at indicated hours post infection. Error bars represent SEM. * = p < 0.05 using the Mann–Whitney U-test.