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Figure 4 | Virology Journal

Figure 4

From: Biphasic regulation of A20 gene expression during human cytomegalovirus infection

Figure 4

Effects of knockdown on HCMV growth. (A) HF cells expressing control shRNA (shC) and shRNA specific for A20 (shA20) were produced by lentiviral vectors. The levels of A20 and β-actin (as a loading control) were shown by immunoblotting. (B and C) Control (shC) and A20-knockdown (shA20) HF cells in 12-well plates were infected with HCMV at an MOI of 0.5 or 3. The growth curves shown represent the total numbers of infectious center-forming units (IFU) (averages of triplicate) produced in cell culture supernatants at the indicated sampling times (B). Total cell lysates were prepared at indicated sampling times and the levels of viral immediate-early (IE1 and IE2), early (p52), and late (pp28) proteins and cellular IκBα, A20, and β-actin proteins were shown by immunoblotting (C).

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