VMir prediction of HAV antigenome-encoded pre-miRNAs. (A) The candidate pre-miRNA with high scores is circled in red. The VMir analyzer program was used to predict candidate pre-miRNAs in the HAV antigenome with a cutoff score of 150 and a cutoff window counts of 35. The y-axis represents VMir scores, while the x-axis indicates the nucleotide position. (B) Secondary structures of the predicted candidate HAV pre-miRNAs. Mature miRNA that was experimentally validated in this study is highlighted and indicated as “novel miR-N1-3p”.