EBNA1 inhibits GKN1 and GKN2 transcription after DNA demethylation in GC cells. (A) AGS cells were transduced with pLU-Vector (Vec) or pLU-EBNA1 and then untreated (Untr) or treated with 10 μM 5′-azacytidine (Aza) for 48 hrs. GKN1 and GKN2 mRNA were quantified by qRT-PCR relative to GAPDH. (B) Fold induction of GKN1 and GKN2 mRNA by Aza were quantified from panel A. (C) qRT-PCR analysis of EBNA1 mRNA levels in AGS cells. (D) Same as in A, except for using MKN74 rather than AGS cells. (E) Fold induction of GKN1 and GKN2 mRNA quantified from panel D. (F) qRT-PCR analysis of EBNA1 mRNA levels in MKN74 cells. *indicates p < .05, **indicates p < .005. Error bars indicated sdm for n = 3.