Skip to main content
Figure 4 | Virology Journal

Figure 4

From: A recombinant adenovirus-based vector elicits a specific humoral immune response against the V3 loop of HIV-1 gp120 in mice through the “Antigen Capsid-Incorporation” strategy

Figure 4

Induction of V3-specific humoral immune response after immunization. A) Schematic of “prime-boost” regimen in C57BL/6 mice. Mice were injected with Ad, Ad-HVR1-lgs-His6-V3 and Ad-HVR1-long-V3, respectively. The solid lines with arrow denote the time of prime and boost, the dotted lines with arrow denote the time of bleeding post injection. B) Sera-based isotype ELISA was employed to determine the levels of V3-specific IgG1 isotype induced in the sera post-boost with vectors Ad, Ad-HVR1-lgs-His6-V3 and Ad-HVR1-long-V3. C) Sera-based isotype ELISA was employed to determine the levels of V3-specific IgG2a isotype induced in the sera post-boost with vectors Ad, Ad-HVR1-lgs-His6-V3 and Ad-HVR1-long-V3. D) Sera-based isotype ELISA was employed to determine the levels of V3-specific IgG2b isotype induced in the sera post-boost with vectors Ad, Ad-HVR1-lgs-His6-V3 and Ad-HVR1-long-V3. The values were expressed as the mean ± standard deviation, representing eight independent replicates.

Back to article page

Virology Journal

ISSN: 1743-422X