Skip to main content
Figure 2 | Virology Journal

Figure 2

From: A recombinant adenovirus-based vector elicits a specific humoral immune response against the V3 loop of HIV-1 gp120 in mice through the “Antigen Capsid-Incorporation” strategy

Figure 2

Evaluation of both the His and V3 exposure capabilities on the capsid surfaces of four versions of hAd5-based V3 vectors. Whole virus ELISA was employed for this purpose. In this method, four versions of rescued vectors were coated in the wells of ELISA plates at various concentrations, starting from 4.5 × 109 VP/well, and serially diluted at 1:2, until an ending concentration of 4.0 × 106 VP/well. A) His exposure was evaluated separately in the vectors: Ad (diamond), Ad-HVR1-lgs-His6-V3 (square), Ad-HVR1-V3 (triangle), Ad-HVR1-long-V3 (dark circle) and Ad-HVR1-lgs-V3-His6-lgs (blank circle). B) V3 exposure was evaluated separately in the same five vectors, with the same order arrangement in Figure 2A. The values were expressed as the means from two replicates.

Back to article page

Virology Journal

ISSN: 1743-422X