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Figure 6 | Virology Journal

Figure 6

From: Effective suppression of Dengue virus using a novel group-I intron that induces apoptotic cell death upon infection through conditional expression of the Bax C-terminal domain

Figure 6

U143ΔN Bax constructs effectively suppresses DENV in transformed mosquito cells. C6/36 cells transformed with anti-DENV group I introns were challenged with the DENV serotypes 1 through 4 (MOI 0.01). At 4 dpi supernatants were collected and viral titers were determined by TCID50-IFA as described in [17]. Suppression of DENV replication is determined through detection of the cell surface expressed DENV E protein. Only in mosquito cells expressing a functional αDENV-U143-ΔN Bax construct were suppression of DENV evident. Suppression of viral replication occurred with all serotypes tested, and was increased when FL was replaced by αDENV-U143-ΔN Bax or αDENV-U143-ΔN Bax-D as the 3-exon. U143-ΔN Bax-D refers to the active αDENV-U143-ΔN Bax construct that is linked to the Drosophila C virus DCV IRES/mCherry configuration as shown in Figure 2. I-Wt = infected naïve C6/36 cells. I-mCh = DENV infected C6/36 cells transformed with a construct constitutively expressing the mCherry fluorescent marker. U = uninfected naïve C6/36 cells. ΔN B = ΔN Bax.

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