Figure 7

Z and R transcript and protein levels are altered by rapamycin treatment. Raji and AGS-BDneo cells were treated with 5 nM rapamycin for 24 hr prior to induction of lytic replication. A. Total RNA was isolated and qRT-PCR was performed with GAPDH (control)-, Z-, or R-specific primers, in triplicate. The mean fold expression over uninduced cells, standardized to GAPDH levels, is presented. B. Z and R protein levels from Western blots (in triplicate) were quantified and standardized to tubulin levels. The resulting Z and R protein levels from AGS-BDneo, Raji, and EBfaV-GFP cells are presented, relative to induced cells (with no rapamycin treatment). * = P < 0.05 relative to induced cells (with no rapamycin treatment); # = P < 0.05 relative to R levels within same cell type.