Figure 6From: Inhibition of mTORC1 inhibits lytic replication of Epstein-Barr virus in a cell-type specific mannerShorter exposure times to rapamycin are not more effective at altering BMRF1 production in either B or epithelial cells. Raji (A) and D98/HR1 (B) cells were treated with 5 nM rapamycin for the times indicated prior to induction. The rapamycin-containing media was washed off the cells at time 0 (the time of induction). Two days post-induction proteins were harvested and Western blot analyses were performed with anti-BMRF1, anti-Z, anti-R, and anti-tubulin antibodies. The resulting bands were quantified and standardized to tubulin levels. Resulting BMRF1 protein levels (middle panels), and Z and R levels (lower panels) are presented, relative to 0Â hr.Back to article page