Higher doses of rapamycin are not effective in inhibiting EBV lytic replication in AGS-BDneo cells. A, AGS-BDneo cells, B, Raji cells. Cells were treated with rapamycin as indicated for 24 hr prior to the induction of lytic replication. Western blot analysis was performed with anti-BMRF1 and anti-tubulin antibodies. The BMRF1 levels were quantified and standardized to tubulin levels. The resulting BMRF1 protein levels are presented, relative to induced cells (with no rapamycin treatment).