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Figure 2 | Virology Journal

Figure 2

From: The placental specific gene, PLAC1, is induced by the Epstein-Barr virus and is expressed in human tumor cells

Figure 2

Expression of PLAC1 in EBV positive vs. EBV negative MutuI cells. a) Schematic representation of two splicing variants for PLAC1 and position of PCR primers used for real time RT-PCR reactions. Primers are designed to span introns to avoid any potential amplification of trace amounts of genomic DNA in RNA preparations. Gels show the amplified products from MutuI cells after 40 cycles. b) Real time RT-PCR analysis of RNA derived from Mutu E1dn bulk vs Mutu Cntl bulk cultures and EBNA1dn infected DG75 (EBV negative) BL cell line. c) Real time RT-PCR analysis of E1dn (EBV negative) and control (EBV positive) clones using primer set 1. d) Real time RT-PCR analysis of E1dn (EBV negative) and control (EBV positive) cells using primer set 2.

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Virology Journal

ISSN: 1743-422X