Molecular epidemiology of Porcine torovirus (PToV) in Sichuan Province, China: 2011–2013

Table 1 Oligonucleotide primers used for the detection of PEDV, TGEV and RVA in fecal samples obtained from pigs with diarrhea

Target viruses^a	Target genes^b	Primer sequence, 5’-3’^c	Size (bp)	Source or reference
PEDV	S	F:TTCTGAGTCACGAACAGCCA	651	[19]
PEDV	S	R:CATATGCAGCCTGCTCTGAA	651	[19]
TGEV	S	F:GTGGTTTTGGTYRTAAATGC	859	[19]
TGEV	S	R:CACTAACCAACGTGGARCTA	859	[19]
RVA	VP6	F: AAAGATGCTAGGGACAAAATTG	308	[20]
		R: TTCAGATTGTGGAGCTATTCCA	308
		nF:GACAAAATTGTCGAAGGCACATTATA	121
		nR: TCGGTAGATTACCAATTCCTCCAG	121

^aPEDV: porcine epidemic diarrhea coronavirus; TGEV: Transmissible gastroenteritis coronavirus; RVA: group A rotavirus.
^bORF: S: spike protein; VP6: viral protein 6.
^cF: Forward primer for RT-PCR; R: Reverse primer for RT-PCR.
nF: Forward primer for nested PCR; nR: Reverse primer for nested PCR.

ISSN: 1743-422X